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cd11b fitc  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd11b fitc
    Cd11b Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+fitc/CD11b+Antibody%2C+anti-human%2C+REAfinity/pm42443171-332-8-10
    Average 95 stars, based on 85 article reviews
    cd11b fitc - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Staining:

    Article Title: The RXR Agonist MSU-42011 Reduces Tumor Burden in a Murine Preclinical NF1-Deficient Model.
    Article Snippet: Cells were passed through a 40 μm cell strainer (BD Falcon, Glendale, AZ, USA), and red blood cells were removed using a lysis solution. .. The resulting single cells were resuspended in Brilliant Buffer (BD Biosciences, Sparks Glencoe, MD, USA) and stained at 4 ◦C for 30 min with the following antibodies: CD4-BV750 (1:100, Biolegend, San Diego, CA, USA, GK1.5), CD25-PE (0.5:100, Biolegend, 3C7), CD8-BV605 (1:100, Biolegend, 104732), FOXP3-PE-Cy5 (1:100, eBioscience, FJK-16s), CD206-BV421 (1:100, Biolegend, C068C2), F4/80-APC-Fire 750 (1:100, Biolegend, BM8), IA-IE-BV650 (0.5:100, Biolegend, M5/114.15.2), CD163-APC (1:100, Biolegend, S15049F), CD45-PerCPCy5.5 (0.5:100, Biolegend, 30-F11), CD11b-FITC (1:100, Miltenyi Biotec, M1/70.15.11.5), CD11c-BV510 (1:100, Biolegend, M1/70), CD19-BV570 (0.5:100, Biolegend, 6D5), CD3Vioblue (5:100, Miltenyi Biotec, 17A2), Viability–LIVE-DEAD Far Red (0.5:100, Thermo Fisher Scientific), and 5 μg/mL anti-mouse CD16/CD32 antibody (Biolegend) to reduce antibody binding to Fc receptors. .. Cells were analyzed using Cytek Aurora and FlowJo v10.0.7r2 software (Tree Star).

    Article Title: Neutrophil myeloperoxidase as a functional biomarker for RSV severity: implications for in vitro therapeutic screening
    Article Snippet: After migration, neutrophils (apical, adherent, basolateral) were collected, centrifuged, blocked with Human TruStain FcXTM, and stained with LIVE/DEADTM fixable violet dye (Thermo Fisher Scientific, UK). .. Following washing, cells were stained with 1/50 dilution of CD11b-FITC (Miltenyi Biotec, 130-110-552), CD64-APC-Cy7 (Miltenyi Biotec, 130-116-199), and CD62L-PE-Cy7 (Miltenyi Biotec, 130-129-810) for 20 minutes at 4°C in the dark. .. Cells were washed, fixed with PFA, permeabilised, and stained intracellularly with MPO-APC (Miltenyi Biotec, 130-119-786) and NE-PE (Santa Cruz Biotechnology, sc-55549 PE).

    Article Title: Microglia regulate motor neuron plasticity via reciprocal fractalkine and adenosine signaling
    Article Snippet: Cell pellets were resuspended in Stain buffer (BD Pharmigen). .. Cells were stained with CD11b-FITC (Miltenyi Biotec) and CX3CR1 (Invitrogen) antibodies in the dark at 4°C for 25 minutes followed by incubation with secondary goat anti-rabbit antibodies conjugated to AlexaFluor405 (Invitrogen) for 25 min at the same conditions. .. Cells were then permeabilized for 15 min using Permeabilization buffer (Invitrogen) and stained with CD39 (Proteintech), GFAP-PE (Miltenyi Biotec) and NF-H-AlexaFluor647 (Novus Biologicals) antibodies followed by staining with the secondary antibodies.

    Article Title: Tumor microenvironment remodeling by STING agonism sensitizes endothelial cells to cytotoxic anti-PD-L1/L2 antibody.
    Article Snippet: Fc receptors were blocked using anti-mouse CD16/CD32 antibody (ThermoFisher Scientific). .. Surface staining was performed at 4 °C for 30 minutes using various combinations of the following fluorophore-conjugated antibodies: CD45-VioGreen, CD11b-FITC, CD11c-APC, F4/80-PE-Vio770, Ly6C-APC/Vio770, Ly6G-VioBlue, CD3-FITC, CD4-APC/Vio770, CD8-VioBlue, PD-1-APC (from Miltenyi Biotec) and CD44-PE, CD206-PE, PD-L1-PE, PD-L1-APC, PD-L1-PeFire700, PD-L2-PE, PD-L2 BUV563 or PD-L2-PECF594 and CD86 (GL1, Brilliant Violet, BV, 711) were from BioLegend. .. B16-PD-L2 or TS/A tumors were harvested, finely minced with sterile blades, and digested at 37 °C for 30 min on an orbital shaker in RPMI-1640 medium containing collagenase I and IV (1 mg/mL each; Merck).

    Article Title: The RXR Agonist MSU-42011 Reduces Tumor Burden in a Murine Preclinical NF1-Deficient Model
    Article Snippet: Cells were passed through a 40 μm cell strainer (BD Falcon, Glendale, AZ, USA), and red blood cells were removed using a lysis solution. .. The resulting single cells were resuspended in Brilliant Buffer (BD Biosciences, Sparks Glencoe, MD, USA) and stained at 4 °C for 30 min with the following antibodies: CD4-BV750 (1:100, Biolegend, San Diego, CA, USA, GK1.5), CD25-PE (0.5:100, Biolegend, 3C7), CD8-BV605 (1:100, Biolegend, 104732), FOXP3-PE-Cy5 (1:100, eBioscience, FJK-16s), CD206-BV421 (1:100, Biolegend, C068C2), F4/80-APC-Fire 750 (1:100, Biolegend, BM8), IA-IE-BV650 (0.5:100, Biolegend, M5/114.15.2), CD163-APC (1:100, Biolegend, S15049F), CD45-PerCP-Cy5.5 (0.5:100, Biolegend, 30-F11), CD11b-FITC (1:100, Miltenyi Biotec, M1/70.15.11.5), CD11c-BV510 (1:100, Biolegend, M1/70), CD19-BV570 (0.5:100, Biolegend, 6D5), CD3-Vioblue (5:100, Miltenyi Biotec, 17A2), Viability–LIVE-DEAD Far Red (0.5:100, Thermo Fisher Scientific), and 5 μg/mL anti-mouse CD16/CD32 antibody (Biolegend) to reduce antibody binding to Fc receptors. .. Cells were analyzed using Cytek Aurora and FlowJo v10.0.7r2 software (Tree Star).

    Article Title: Neutrophil myeloperoxidase as a functional biomarker for RSV severity: implications for in vitro therapeutic screening.
    Article Snippet: Assessment of neutrophil markers and degranulation After migration, neutrophils (apical, adherent and basolateral) were collected, centrifuged, blocked with Human TruStain FcX (#422302, BioLegend, USA), and stained with LIVE/DEAD fixable violet dye (#L34963, Thermo Fisher Scientific, UK). .. Followingwashing, cells were stained with 1/50 dilution of CD11b-FITC (#130-110-552, Miltenyi Biotec), CD64-APC-Cy7 (#130-116-199,Miltenyi Biotec), andCD62L-PE-Cy7 (#130-129-810, Miltenyi Biotec) for 20min at 4 °C in the dark. .. Cells were washed, fixed with PFA, permeabilised and stained intracellularly with 1/50 dilution of MPO-APC (#130-119-786, Miltenyi Biotec) and NEPE (#sc-55549 PE, Santa Cruz Biotechnology).

    Binding Assay:

    Article Title: The RXR Agonist MSU-42011 Reduces Tumor Burden in a Murine Preclinical NF1-Deficient Model.
    Article Snippet: Cells were passed through a 40 μm cell strainer (BD Falcon, Glendale, AZ, USA), and red blood cells were removed using a lysis solution. .. The resulting single cells were resuspended in Brilliant Buffer (BD Biosciences, Sparks Glencoe, MD, USA) and stained at 4 ◦C for 30 min with the following antibodies: CD4-BV750 (1:100, Biolegend, San Diego, CA, USA, GK1.5), CD25-PE (0.5:100, Biolegend, 3C7), CD8-BV605 (1:100, Biolegend, 104732), FOXP3-PE-Cy5 (1:100, eBioscience, FJK-16s), CD206-BV421 (1:100, Biolegend, C068C2), F4/80-APC-Fire 750 (1:100, Biolegend, BM8), IA-IE-BV650 (0.5:100, Biolegend, M5/114.15.2), CD163-APC (1:100, Biolegend, S15049F), CD45-PerCPCy5.5 (0.5:100, Biolegend, 30-F11), CD11b-FITC (1:100, Miltenyi Biotec, M1/70.15.11.5), CD11c-BV510 (1:100, Biolegend, M1/70), CD19-BV570 (0.5:100, Biolegend, 6D5), CD3Vioblue (5:100, Miltenyi Biotec, 17A2), Viability–LIVE-DEAD Far Red (0.5:100, Thermo Fisher Scientific), and 5 μg/mL anti-mouse CD16/CD32 antibody (Biolegend) to reduce antibody binding to Fc receptors. .. Cells were analyzed using Cytek Aurora and FlowJo v10.0.7r2 software (Tree Star).

    Article Title: The RXR Agonist MSU-42011 Reduces Tumor Burden in a Murine Preclinical NF1-Deficient Model
    Article Snippet: Cells were passed through a 40 μm cell strainer (BD Falcon, Glendale, AZ, USA), and red blood cells were removed using a lysis solution. .. The resulting single cells were resuspended in Brilliant Buffer (BD Biosciences, Sparks Glencoe, MD, USA) and stained at 4 °C for 30 min with the following antibodies: CD4-BV750 (1:100, Biolegend, San Diego, CA, USA, GK1.5), CD25-PE (0.5:100, Biolegend, 3C7), CD8-BV605 (1:100, Biolegend, 104732), FOXP3-PE-Cy5 (1:100, eBioscience, FJK-16s), CD206-BV421 (1:100, Biolegend, C068C2), F4/80-APC-Fire 750 (1:100, Biolegend, BM8), IA-IE-BV650 (0.5:100, Biolegend, M5/114.15.2), CD163-APC (1:100, Biolegend, S15049F), CD45-PerCP-Cy5.5 (0.5:100, Biolegend, 30-F11), CD11b-FITC (1:100, Miltenyi Biotec, M1/70.15.11.5), CD11c-BV510 (1:100, Biolegend, M1/70), CD19-BV570 (0.5:100, Biolegend, 6D5), CD3-Vioblue (5:100, Miltenyi Biotec, 17A2), Viability–LIVE-DEAD Far Red (0.5:100, Thermo Fisher Scientific), and 5 μg/mL anti-mouse CD16/CD32 antibody (Biolegend) to reduce antibody binding to Fc receptors. .. Cells were analyzed using Cytek Aurora and FlowJo v10.0.7r2 software (Tree Star).

    Incubation:

    Article Title: Microglia regulate motor neuron plasticity via reciprocal fractalkine and adenosine signaling
    Article Snippet: Cell pellets were resuspended in Stain buffer (BD Pharmigen). .. Cells were stained with CD11b-FITC (Miltenyi Biotec) and CX3CR1 (Invitrogen) antibodies in the dark at 4°C for 25 minutes followed by incubation with secondary goat anti-rabbit antibodies conjugated to AlexaFluor405 (Invitrogen) for 25 min at the same conditions. .. Cells were then permeabilized for 15 min using Permeabilization buffer (Invitrogen) and stained with CD39 (Proteintech), GFAP-PE (Miltenyi Biotec) and NF-H-AlexaFluor647 (Novus Biologicals) antibodies followed by staining with the secondary antibodies.

    other:

    Article Title: Adipose tissue from oesophageal adenocarcinoma patients is differentially affected by chemotherapy and chemoradiotherapy regimens altering immune cell phenotype and cancer cell metabolism
    Article Snippet: Antibodies used for DC staining included CD40-FITC, CD11c-Vio-Blue, CD80-PE, CD83-APC-Vio770, CD86-PerCp-Vio700, HLA-DR-Vio-Green, CD54-APC, and PD-L1-PE-Vio770.

    Article Title: Adipose tissue from oesophageal adenocarcinoma patients is differentially affected by chemotherapy and chemoradiotherapy regimens altering immune cell phenotype and cancer cell metabolism.
    Article Snippet: Oesophageal adenocarcinoma (OAC) is a poor prognosis cancer with limited responses to standard of care treatments including chemotherapy and chemoradiotherapy.. OAC has one of the strongest associations with obesity, its anatomical location surrounded by visceral adipose tissue has been postulated to intensify this association.. Adipose tissue is a regulatory organ with many unknown downstream functions, including its direct response to chemotherapy and radiotherapy.



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